ملخص البحث :

The aim of this study was to evaluate the polymorphism relationship of CYP19 exon4 gene and its effect on the concentration of estrogen hormone and pregnancy in local Awassi ewes. 50 ewes of the local Awassi sheep were used in the Husayniyah threshold fields located on the Najaf, Karbala road, in Iraq, during a period from September of 2019 to January of 2020. Blood samples from Awassi sheep were used for DNA extraction and detection of CYP19 gene by PCR and serum samples were used for measurement concentration of estrogen and progesterone. The highest concentration in the hormone (16.33 pg/ml), the lowest concentration (9.38 pg/ml), the highest concentration in the hormone pregnancy (0.58 ng/ml) and the lowest concentration (0.13 ng/ml) were isolated . CYP19 gene exon 4 of the 105 bp gene and the identification of genotypes, depending on the neo-sequence The distribution of CYP19 genotypes in local Awassi ewes specimens was 20%, 30% and 50% for TT, TC and CC genotypes respectively. P<0.05) in TT and TC genotypes (15.57,15.46) respectively, compared to CC (14.23), while there was no significant difference between TT and TC genotypes. P<0.05 in genotypes TC (0.47) on TT and CC genotypes (0.32,0.24) while increased pregnancy hormone genetic model TT significant decrease compared to genetic Btrz TC and CC. The current study revealed that the TC genotype of CYP19 gene was prevalent on the TT and CC genotypes of Polymorphism of the CYP19 gene in Exon4. Keywords: Exon4, CYP19 Gene, Local Awassi ewes, Hormonal Tonic and Pregnancy.

ملخص البحث :

Abstract This study was carried out to investigate the effect of the genetic polymorphism of the third exon for CYP19 gene on the quantity and composition of milk in local female goats. Blood samples were collected from female goats in the fields of some breeder in Babil Governorate/ Al-Qasim City. Milk composition were examined in the Public Health Laboratory at Al-Qasim Green University/College of Veterinary Medicine, the genetic bundle of the third axon of the CYP19 gene was isolated, which was 140 bp in size, and its genotyping was determined according to the different nucleotide sequences and their association with the quantity and milk composition in the samples which tested in the Bayolat Laboratory in Al-Diwaniyah Governorate. The proportions of the distribution of the genotypes of the CYP19 gene in a sample of local female goats TT, GG and TG were 33.34%, 26.66 and 40%, respectively. The results showed prevalence of the TG genotype for CYP19 gene in the Iraqi local goats on the pure genotypes TT and GG, which was a highly significant superior (P<0.01) in the percentages of fat, protein, lactose and CFPP, as well as the significant superiority of the mixed genotype significantly on the pure genotype in the percentages of solid non-fat and density. It could be concluded from the current study that the selection of females with TG genotype as source for future generations to obtain a generation selected for the desired traits. Key words: Exon3, CYP19 gene, Local female goats, Milk composition