ملخص البحث :

ABSTRACT : The purpose of this study was to investigate some pathogenesis aspects in puppies after experimental infection with G. duodenalis of 10 local breed dogs, age 4 months as experimental infected dogs and 5 dogs as control. A clinical signs of experimental infection of puppies showed mild increased in the temperature and respiratory rate after one week of infection. The results of biochemical tests showed significant increas in the levels of blood sugar and decreased in the insulin and amylase level; significant decreased in the levels of serum cholesterol, triglycerides, VLDL, LDL and HDL, while alkaline phosphatase showed significant increased.The measurement of antibodies concentration revealed that the significant decreased in the concentration of the IgA was found in the experimental infected dogs compared with control dogs, while no significant differences was found in the concentration of the IgG.The measurement of antioxidant concentration demonstrated a significant decreased in the levels of glutathione and superoxide dismutase in the duodenum, liver, gall bladder and pancreas in infected dogs compared with non infected dogs.A different histopathological changes was found induodenum, Pancreas, gall baldder and liver. Internal tissue (duodenum, pancreas, liver and gall bladde) were Immunohistchemically evaluated for two receptors , which were tumor nectosis factor – alpha (TNF-alpha) and interluken- 6 (IL-6). Tumor nectosis factor – alpha receptor was positive in 80% of doudenum , 60% of pancreas, 50% of gall bladder and 30% of liver of the expermintal infected dogs. Duodenum postive (70%) for IL-6 more frequent than of the panceas, liver and gall bladder (60%, 20% and 60%, respectivly).