ملخص البحث :

determine the Cryptosporidium spp in cattle in different parts of Baghdad province. A total number of 100 fecal samples from different age groups were collected. Conventional method using modified Ziehl-Neelsen for staining fecal smears and molecular techniques for detection the prevalence and determines the species of Cryptosporidium that cause infection. The overall prevalence of infection with Cryptosporidium was 21%(21/100) by conventional method; nested PCR was done that targeting 18S rRNA gene on the same samples in which Cryptosporidium DNA identified in 38 samples (38%). Four species of Cryptosporidium in cattle were detected for the first time in Baghdad province: C. parvum (6/10), C. andersoni (2/10), C. bovis (1/10) and C. ryanae (1/10). The determination and characterization of Cryptosporidium spp in cattle was very important to avoid the infection to other animals and handlers and for applying control programs.

ملخص البحث :

stage of tapeworm Echinococcus granulosus. This disease is one from zoonotic disease that transmitted from animals to human. The primary definitive hosts for this parasite are Dogs and other canids and the intermediate hosts are sheep, cattle, goats, pigs or camels while human is an accidental intermediate host of echinococcosis that take the infection by ingestion of eggs that lies with the feces’ of infected dogs

ملخص البحث :

Prevalence and strains of Cryptosporidium detected by microscopy and PCR in stools of cattle handlers, Onl J Vet Res., 22 (12):1095-1102, 2018. Authors report prevalence and species of Cryptosporidium in stools of 100 volunteer cattle handlers aged <10 - to >40 years determined by microscopy and nPCR, collected during January to September 2018 in Iraq. We found oocysts in 17% by microscopy, but by PCR detected Cryptosporidium in 41% of stool samples. By PCR, children aged < 10 had a very high incidence of 69.9% (P≤0.05), 19-20 year old ~30%, and those over 40 years ~18%. There was no significant difference in presence of Cryptosporidium between genders. By PCR with 18S rRNA gene of 10 stool samples phylogenetic tree of our C. parvum, C. hominis and C. andersoni isolates referenced against Gen-Bank revealed 5 closely related to Cryptosporidium andersoni (KX710086.1) 3 to Cryptosporidium hominis (KT123173.1), and 2 to Cryptosporidium parvum isolates (MH341586.1) with a genetic difference of 0.01-0.04%. Datasets suggested strong genetic distinctiveness amongst species. C. andersoni has not been reported previously in this region in dairy/cattle handlers but C. parvum causes most infection.