ملخص البحث :

n everyday practice, breast soft tissue lesions frequently provide a diagnostic problem. They are roughly separated into malignant and benign tumor lesions, which cover a wide range of histopathological abnormalities. This study's objective is to measure the levels of IL1 and IL1 in patients with breast cancers' serum and tissue. an example of In the period from November 2021 to November 2022, breast tissue was taken from 70 female patients (aged 14 to 66) having breast surgery in Babylon Province at AL-Hilla Teaching Hospital and AL-Fayhaa National Hospital. The women underwent lumpectomies or mastectomies for benign or harmful tumors; some had a history of breast cancer, while others did not. The tissue that was gathered for analysis was combined 5 cm outside the tumor's margin in the case of females who had tumors. Fresh tissue was immediately put in a sterilized plane tube or pee cup after circumcision. It was cut, homogenized, and had a standard saline solution. and mixed with wooden sticks using a sterile surgical knife. 30 minutes after collection Using an enzyme-linked immunosorbent assay, IL1 and IL1 were evaluated in the serum and tissue of both healthy and breast tumor-bearing women (ELISA) The results showed that the mean of IL1 in serum of breast cancer was 1.052 and the mean of IL1 in Benign breast tumor was 1.208 that increased significantly in patients compared with control. Additionally, the mean of IL1 in serum of breast cancer was 5.287 and the mean of Benign breast tumor was 4.993 that did not increase significantly compared with control, and the mean of IL1 beta in locally was 21.000 increase significant than the mean of IL1.

ملخص البحث :

A tumor is a mass of abnormal tissue. There are two types of breast cancer tumors: those that are non-cancerous, or ‘benign’, and those that are cancerous, which are ‘malignant’ Seventy (70) biopsy of breast tumors patient's women without chemotherapy for DNA extraction for genetic polymorphism of TLR2 and local immunity study for TLR2, fifty (50) blood sample from healthy women as control group. One hundred (100) blood sample from women with breast tumor for polymorphism of TLR2 and evaluated immune markers (TLR2).The age of women in this study ranging from (14-66) years in AL-Hilla-Teaching hospital , Imam Sadiq Hospital and Marjan hospital a period from September 2021 to October 2022.the result appeared that By Enzyme Linked Immunosorbent Assay technique showed that the concentration of TLR2 in serum patients were (9.953±4.606) significantly increased compare with control (6.774±3.855 ) respectively, The concentrations of TLR2 in serum patients were (5.599±3.550) significantly different compare with concentrations tissue (21.000 ±14.356 )respectively , For TLR2 196 to 174 del genotyping the result was significantly only deletion in (G, C , T ) between patients with chemotherapy and control ) at (p =0.003* , 0.0007* , 0.04*) respectively , also the result significantly for insertion of (G) between patients with chemotherapy and control ) at (p =0.01* ) conclusion of this research TLR2 196 to 174 del genotyping the result was significantly only deletion in (G, C , T ) between patients with chemotherapy and control ) at (p =0.003* , 0.0007* , 0.04*) respectively , also the result significantly for insertion of (G) between patients with chemotherapy and control ) at (p =0.01*