ملخص البحث :
Collected 180 samples of urine from reviewers patients to Diwaniya Teaching Hospital and
Women's Hospital and children education in the city of Diwaniya, for the period from 01/09/2015
until 31/01/2016 and both sexes, To isolate the bacterium Staphylococcus spp. that causes
inflammation of the urinary tract and to determine the virulence factors phenotypic and molecular, It
found that the rate of 120 (66.66%) samples gave moral growth of bacteria, while 60 increased by
(33.33%) a negative sample. It has been found that the isolation of 24 belonging to the bacteria
Staphylococcus spp. Distributed on 20 isolated by (16.67%) belonging to the bacteria Staphylococcus
aureus and 4 isolates by (3.33%) belonging to the bacteria Staphylococcus haemolyticus of the total
samples positive for culture, The isolates producing hemolysin and hemolytic has included all isolates of
Staph. aureus and Staph. haemolyticus, Using polymerase chain reaction technique (PCR) proved
that genes hlB and hlA exist in bacteria Staphylococcus spp. They encoded to produce hemolysin
as it was characterized by all isolates of Staph. aureus analyst blood its proximity to the gene hlA
by (100%) and the gene hlB by (40%), when you make detection of genes hemolysin in Staph.
haemolyticus appears having gene hlA the rate of (75%) and gene hlB which increased by 25%.
The investigation of some virulence factors associated with pathodenicity of bacterial isolates
analyst for the blood which included the formation capsule, agglutination of red blood cells and
the production of biofilm. produced isolates Staph. aureus and Staph. haemolyticus capsule
(40%, 25%) respectively the results of statistical analysis showed no significant differences
between them at the level of (P> 0.05), As for the ability of the isolates the agglutination of red
blood cells marked by Staph. aureus ability to events agglutination and by (30%) while the all
isolates of Staph. haemolyticus unable to events agglutination the results of statistical analysis
showed that there were significant differences at the level (P<0.05). also showed bacterial
species, an analyst for the blood the ability to produce biofilm at different rates, As it reached
isolates producing biofilm ratio (45% and 50%) of bacteria Staph. aureus and Staph. haemolyticus respectively.
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سنة النشر : 2017
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تصنيف البحث : other
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