ملخص البحث :

This study aimed to prepare an experimental nano vaccine for Acinetobacter baumannii. In current study, Acinetobacter isolates were identified by growth onto CHRO Magar TM Acinetobacter/MDR and standard biochemical tests. Identification of isolates of Acinetobacter isolates to species level was done by VITEK 2 compact system and API20. Chitosan nanoparticles was used in this study as an adjuvant, the results showed chitosan a perfect adjuvant because it carried outer membrane protein antigen and stimulates an immune response at treated rats, use of Chitosan nanoparticles as a carrier to OMP increased the concentration of cytokines (IL-2, IL-6, IFN-ƴ), antibody titer, total leukocytes and differential leukocytes in treated rats compared with each control group and OMP group.

ملخص البحث :

Because of the strong humoral immune response that accompanies H. pylori infection, serological detection of anti-H. pylori antibodies, particularly IgG, have been considered as a reliable tool for diagnosing recent infection as well as assessing chronic infectious state. Actually, little has been mentioned, about the value of assessing IgM before and after a proper course of antibiotic therapy against H. pylori. in the current study we tried to evaluate such role by measuring serum IgM before and after anti-H. pylori therapy in a group of Iraqi patients with documented H. pylori infection. The current prospective study was carried out in Adiwaniyah province, a midEuphrates region of Iraq and included 317 patients with dyspeptic symptoms with high clinical suspicion of H. pylori. The age range of those patients was from 19 to 67 years and the study included 200 male and 117 female patients. Those patients were treated with anti-H. pylori antibiotic regimen according to medical textbook guidelines and before starting treatment a venous blood sample was sent to the laboratory in order to measure the level of antH. pylori IgM antibody. After the end of a single course of antibiotic therapy, every patient was subjected to another serological test of the IgM level. The IgM level was assessed by ELISA according to the instruction of the providing company. The study included 317 patients, 200 men and 117 women with a mean age of 35.61 ±10.13 years. Most of the patients were from urban areas. The majority of patients were employed or students; however, a substantial proportion of them was unemployed or retired. Illiteracy accounted for a substantial proportion of the study sample 25.6 %. The mean body mass index was 26.51 ±7.81 kg/m2 with a range of 21.03 – 39.91 kg/m2. The mean serum IgM level was 55.22 ±16.50 U/ml before treatment and has been reduced to 36.75 ±6.05 U/ml after treatment; the reduction was highly significant (P < 0.001). It appears that the serological evaluation of serum IgM before initiation of anti-H. pylori antibiotic course and after completing that course is a promising method to assess treatment response; however, much research work is needed to get a clear consensus about such a role.

ملخص البحث :

A number of reports have linked iron deficiency anemia to H. pylori infection. This microorganism is extremely common worldwide and it is estimated to affect 50 % of population with some variations in the prevalence rates attributed to geographic, sociodemographic and other factors; nevertheless, it appears to be more common in developing countries in comparison with developed countries. In developing countries, H. pylori is estimated to affect approximately 65 % of children. The aim of the current study was to shed light on the possible association between H. pylori infection and iron deficiency anemia in children in our Iraqi community. The current study included 34 children who were recruited from the pool of children visiting the consultation unit of pediatric in Maternity and Children Teaching hospital in Al-Diwaniyah Province, Iraq. Some of patients were also recruited from a number of private clinics. Those children were serologically diagnosed to have H. pylori infection. Complete blood count was carried out in addition to assessment of serum ferritin. The beginning of the study is dated back to December the 2nd 2018 and ended on November the 15th 2019. The mean hemoglobin (Hb) concentration was 9.64 ±1.02 g/dl and it ranged from 7.1 -10.9 g/dl; the frequency of anemic patients was 100.0 % based on the results of Hb in comparison with reference values. Besides, the mean mean corpuscular volume (MCV) was 71.94 ±7.82 fl and it ranged from 53.6 -84.5 fl; the frequency of patients with microcytic red blood cells (RBC), MCV < 77 fl, was 67.6 %. In addition, the mean mean corpuscular hemoglobin (MCH) was 20.97 ±3.01 pg and it ranged from 13.6 -27.1 pg; the frequency of patients with hypochromic RBCs, MCH < 25 pg, was 91.2 %. Moreover, the mean mean corpuscular hemoglobin concentration (MCHC) was 229.13 ±1.49 g/dl and it ranged from 25.4 -32.6 g/dl; the frequency of patients with hypochromic RBCs, MCHC < 31 g/dl, was 88.2 %. The mean serum ferritin of contributing children was 28.05 ± 18.05 ng/ml and it ranged from 8.6 -71.5 ng/ml; none of participating children had a serum ferritin level of < 7. children with iron deficiency anemia should be investigated for H. pylori even in the albescence of significant gastrointestinal manifestation because of the apparent association between this microorganism and iron deficiency anemia

ملخص البحث :

A number of reports have linked iron deficiency anemia to H. pylori infection. This microorganism is extremely common worldwide and it is estimated to affect 50 % of population with some variations in the prevalence rates attributed to geographic, sociodemographic and other factors; nevertheless, it appears to be more common in developing countries in comparison with developed countries. In developing countries, H. pylori is estimated to affect approximately 65 % of children. The aim of the current study was to shed light on the possible association between H. pylori infection and iron deficiency anemia in children in our Iraqi community. The current study included 34 children who were recruited from the pool of children visiting the consultation unit of pediatric in Maternity and Children Teaching hospital in Al-Diwaniyah Province, Iraq. Some of patients were also recruited from a number of private clinics. Those children were serologically diagnosed to have H. pylori infection. Complete blood count was carried out in addition to assessment of serum ferritin. The beginning of the study is dated back to December the 2nd 2018 and ended on November the 15th 2019. The mean hemoglobin (Hb) concentration was 9.64 ±1.02 g/dl and it ranged from 7.1 -10.9 g/dl; the frequency of anemic patients was 100.0 % based on the results of Hb in comparison with reference values. Besides, the mean mean corpuscular volume (MCV) was 71.94 ±7.82 fl and it ranged from 53.6 -84.5 fl; the frequency of patients with microcytic red blood cells (RBC), MCV < 77 fl, was 67.6 %. In addition, the mean mean corpuscular hemoglobin (MCH) was 20.97 ±3.01 pg and it ranged from 13.6 -27.1 pg; the frequency of patients with hypochromic RBCs, MCH < 25 pg, was 91.2 %. Moreover, the mean mean corpuscular hemoglobin concentration (MCHC) was 229.13 ±1.49 g/dl and it ranged from 25.4 -32.6 g/dl; the frequency of patients with hypochromic RBCs, MCHC < 31 g/dl, was 88.2 %. The mean serum ferritin of contributing children was 28.05 ± 18.05 ng/ml and it ranged from 8.6 -71.5 ng/ml; none of participating children had a serum ferritin level of < 7. children with iron deficiency anemia should be investigated for H. pylori even in the albescence of significant gastrointestinal manifestation because of the apparent association between this microorganism and iron deficiency anemia