ملخص البحث :

The aim of this study is to determine the mutation in fenugreek genotype using ISSR markers . A molecular study was conducted at molecular lab belongs to biology department in Faculty of Science / University of Kufa to study variation in fenugreek in response to gamma ray exposure. Seeds were irradiated at five doses (0 Gy,25 Gy, 50 Gy, 100 Gy, 150 Gy, 300 Gy and 400 Gy) , at average of 18 Gy/hour using Cobalt 60 as irradiation source ,in addition to not irradiated control treatment. Molecular variation detection accomplished by using sixteen inter simple sequence repeat markers (ISSRs). Data analysis of sixteen ISSR markers revealed that higher 1750bp and lower 136 bp molecular size were produced in primer HB12 and primer UBC849 gave highest value for (main, polymorphic, unique ) bands in addition to discriminatory value .the most efficient primer was UBC-852. Primer UBC881 produced higher value for polymorphism. Higher value for number of amplified produced by primer 17889A. Results indicate the ability of gamma ray in generating of mutation and efficiency of ISSR marker in detecting these mutations.

ملخص البحث :

A novel epidemic infection Coronavirus-19 (COVID-19) considered as one of the challenges in sustainable development. A new-onset of hyperglycaemia has been observed between many COVID-19 patients. The clear explanation of this elevation in fasting plasma glucose (FPG) was debuted. Here we investigate whether this increase is due to impaired insulin secretion or insulin resistance. 269 participants, group 1 (control, n = 46) group 2 (COVID-19 patients, n = 223). 27 Patients were excluded due to missing of their FPG results. FPG, liver enzymes (ALT, AST, and Alk. Phosphatase), b.urea, s.creatinine, s. insulin, C-peptide, D-dimer, and s.ferritin were measured. Our results showed that FPG was increased in 82% (161) patients and this increase was positively correlated with ferritin (r20.039, P-value 0.0013). There is no correlation between FPG with liver enzymes (ALT and AST). The level of insulin hormone and c-peptide were normal. Because there were no increase in insulin or c-peptide and the only relationship was between FPG and ferritin. Therefore, we concluded that COVID-19 infection could cause insulin resistance