ملخص البحث :

One hundred samples (blood, follicular fluid, cervical mucus) was collected from cows suspected with cystic ovary at time of slaughter for a period from sept. 2017 to sept. 2019 in Aldora slaughtering center / Baghdad. All samples centrifugation 3000 rpm / min. then refrigerator at -20 ºC until analysis with biochemical test, MDA measured by Yagi method Glutathione measured by enzymatic recycling method. Catalase assessed according to method of Chohen et al. (1970) Vitamin C and vitamin E measured by idometric and automated method respectively. The results of this study showed significant decreasing P <0.001, P <0.05 of antioxidant in samples of cystic ovary cows compared with ovulated. While the concentration of MDA (stress biomarker) was increase significantly P<0.001 in blood and cervical secretion except the follicular fluid showed significant decreasing P<0.001 in cystic ovary cows compared with ovulated. The results appeared that cystic ovary is a stress state illustrated by disruption of oxidant / antioxidant balance compared with ovulated cows the concentration of antioxidant and oxidative marker was alter according to their importance in the process of ovulation events. Keywords: Oxidative stress, bio markers, blood follicular, ovarian follicular cyst.